How do I detect all possible mutations?

First, under Process -> Options, choose the Mutation tab, then click on "High Sensitivity". Second, analyze with 1D parameters, by clicking on the  icon in the toolbar and ensuring that the colors are gray and blue, rather than red and blue , which indicates 2D settings. By using high sensitivity and 1D settings, you will detect mutations in areas of high noise, although you will need to review the called mutations because you may have more false positives using this method.