Kyle Gabel
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Recent activity by Kyle Gabel-
How do I add forward and reverse samples?
Data analysis is most accurate when both forward and reverse sequence traces are used. I. Software assumes that the filenames with only _F and _R differences are from the same sample. II. If the fi...
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Which types of files do I input?
There are a few options for data input: I. If you do not have a GenBank sequence for the gene of interest, simply enter your trace samples in the Sample Files window; the software will automaticall...
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Can the software detect somatic mutations
Yes the software can detect somatic mutations. Prior to running the data go to Process>Options>Display and check the box labeled, "Check 2D Small Peaks (Mosaic)." The software will place a green li...
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What are the advantages of the software?
The software has many advantages for mutation analysis: Detects real mutations and ignores base calling errors Simple, fast process through automated analysis Accuracy of 99% in detecting mutati...
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What is the procedure to produce results?
There are only three simple steps to produce results using the automatic software:I. Data Entry II. Automated analysisIII. Reporting
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What types of mutations does the software detect?
The software detects homozygous and heterozygous point mutations and indels.
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What does Mutation Surveyor do?
Mutation Surveyor detects mutations and SNPs with DNA trace data resulting from Sanger sequencing. The variations of sample DNA sequence traces are compared to wildtype and GenBank sequence traces.
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How Do I Import Mutation Surveyor Data into Geneticist Assistant?
Follow our guide HERE to for help importing your Mutation Surveyor data into Geneticist Assistant.
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What is the calculation for the Phred-like Score?
Phred-like Score Characteristics Estimates a probability of error for each base-call, as a function of certain parameters computed from the trace data. These error probabilities are to validate ba...
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How do I download a GenBank file?
Use our Downloading a GenBank file from NCBI guide to help you download a GenBank file.