Kyle Gabel

To view comments in the Output Tables Go to Process >> Options >> Output and check the Comments box.

To group sample files for whole gene analysis, open the 2D Output Table and click the Whole Gene Output Icon. Select Filename Match and enter desired characters. This function will group samples wi...

First, under Process -> Options, choose the Mutation tab, then click on "Medium Sensitivity". Second, analyze with 2D parameters, by clicking on the  icon in the toolbar and ensuring that the color...

First, under Process -> Options, choose the Mutation tab, then click on "High Sensitivity". Second, analyze with 1D parameters, by clicking on the  icon in the toolbar and ensuring that the colors ...

You may exclude poor quality data from the report either before or after data analysis. To exclude prior to analysis, go to Process>Options>Output and set the Lane Quality Threshold to 5 for exampl...

Since the software remembers the last used settings, it is best to click the default settings for all tabs under Process >> Options. This will prevent the software from missing mutations as a resul...

The software deletes false positive mutations when they exist only in 1 direction with lower local quality than the other direction for 2 directional data.

The software adds mutations in two different instances:I. When the dropping factor of a mutation is > .15 and the intensity is >500, yet the score does not meet the detection threshold of 5.00, the...

In order to prevent the software from missing mutations, it is important to specify the directional parameters of the analysis by clicking the 2 Directional icon  if you are using bi-directional da...

Scroll through each mutation and delete those that only appear in one direction (Forward or Reverse) and not in both directions. Mutations that appear in both directions and are written in blue are...