Kyle Gabel
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Articles
Recent activity by Kyle Gabel-
What are the computer hardware requirements for MaSTR?
MaSTR Server Minimum Specs (complex mixtures may not run): 4-core CPU 16 GB of RAM 1 TB hard drive MaSTR Server Suggested Specs: 8-core CPU (or better) 32 GB of RAM (or better) 2 TB hard drive (or ...
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What raw data file types are accepted by the software?
.fsa, .hid, .sg1
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What CE instruments and chemistries is GeneMarker compatible with?
GeneMarker software is compatible with output files from all major CE sequencing systems, including ABI®PRISM, Applied Biosystems SeqStudio™, and Promega Spectrum Compact CE Systems genetic analyze...
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Why is the HGVS nomenclature not correct for many variants after an update to Geneticist Assistant?
For all variants, run these commands: cd "C:\Program Files\Softgenetics\Softgenetics Server\ga" venv\Scripts\activate python manage.py calculatevarianthgvs --all if python manage.py calculateva...
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When should I use the BasePatch setting?
If mutation calls are being missed near the beginning of the traces (first 50bp) due to poor mobility shifting, then try running the project with BasePatch selected. Located on the Contig tab of t...
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What raw data file types are accepted by the software?
fsa, .hid, .rsd, .esd, .scf
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Why doesn’t Geneticist Assistant software open?
Reference directory may be incomplete or inaccessible. “C:\ProgramData\SoftGenetics\Geneticist Assistant.ini” [General] reference_directory=C:\\Users\\Public\\Documents\\SoftGenetics\\References\\g...
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What is the function of the “Exclude 1st Base Difference” setting?
This option in the Contig settings is designed to help separate out similar amplicons into different contigs. For optimal performance, contigs should consist of trace files amplified from the same...
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What is the Lane Quality and how do I interpret the value?
The Lane Quality is a measurement of the average signal to noise ratio for the entire lane, or trace. A Lane Quality score of 20 signifies that there is 5% noise in that lane (n/s = 1/20 = .05).
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How is the mutation score calculated for each peak?
Our mutation score is from the theoretical calculation of signal to noise ratio with the normal distribution. Signal is defined as a peak in the mutation electropherogram and noise is defined as th...