-The cause is most likely due to highly saturated ion front; a long stretch in the dye at low MW where it’s flat and around 32,700 RFU.
-After analysis run in software (ChimerMarker, GeneMaker or GeneMarker HID), the electropherogram for one or more of the dye channels displays a negative Y-axis range at the low MW that slopes upward to 0 as shown below.
1.Select samples from Raw Data Folder (A) to view data in main screen or select Raw Data folder (A) then view raw data in All Color browser (B).
2. Zoom into the area between the ion front and the first ILS peak
3. Take note of the raw data frame number (X-axis) in this region.
4. Return to the Run Wizard, second window (Data Process Options)
5. Uncheck Auto-Range (frame) and enter the number noted in step 3 for the Start value.
-Now the data appears normal, and the analysis can proceed as normal using this setting.